Table 4 Characteristics of the investigated markers
Gene | rs | Primers sequences (5′–3′) | Polymorphism | Amplicon (bp) | Fluorochrome |
|---|---|---|---|---|---|
IL1A | 3783553 | F-TGGTCCAAGTTGTGCTTATCC R-ACAGTGGTCTCATGGTTGTCA | INDEL—4pb | 230–234 | 6-FAM |
IL4 | 79071878 | F-AGGGTCAGTCTGGCTACTGTGT R-CAAATCTGTTCACCTCAACTGC | VNTR—70 pb | 217–287 | HEX |
NFKB1 | 28362491 | F-TATGGACCGCATGACTCTATCA R-GGCTCTGGCTTCCTAGCAG | INDEL—4pb | 156–160 | 6-FAM |
PAR1 | 11267092 | F-AAAACTGAACTTTGCCGGTGT R-GGGCCTAGAAGTCCAAATGAG | INDEL—13pb | 265–277 | HEX |