Fig. 3

BMS-754807 reduces wound closure of lung cancer cells. Scratch wounds were made on near confluent plates of lung cancer cells. Cells were treated with BMS-754807 every 24 h, and wound closure was measured over 48–72 h. Representative images from A549 cells at 0 h (a, c) or 48 h (b, d) after treatment with either an appropriate concentration of DMSO or 0.5 μM of BMS-754807. Quantification of the relative scratch wound closure at 48 h in A549 (e) or at 72 h in NCI-H358 (f) cells was determined by measuring the remaining area not filled with cells and presenting this relative to the area of the scratch at time 0 h. Data represents mean ± SEM (n = 4), *p < 0.05 as determined by a paired Student’s T-test