Fig. 4

Genome walking identification of integration sites using CBD 1 gene and vector specific forward primers and four sets of ApaGene reverse primers labeled A, B, C and D. After three rounds of PCR a single band was evident for B7, while multiple bands were present in the B48 samples. Band sizes greater than 500 bp are needed to identify integration sequences. Large bold sequence represents the pBI121 T-DNA border region, underlined sequences represent unique potato sequence, remaining sequence is common to APAgene kit