Table 1 Primers used for the amplification of the large (L) RNA of Tomato spotted wilt virus WA-USA (TSWV WA-USA) isolate
Oligonucleotides | Sequence (5′-3′) | Position in L RNA | Expected ampliconsize (bp) |
|---|---|---|---|
TSWVF1 | AGAGCAATCAGGTAACAACG | 1 | 2782 |
TSWVR1 | AATCGGACAGTGGCAAAAAC | 2782 | |
TSWVF2 | GCGCAATTTTCAAAGAAAGC | 2572 | 1822 |
TSWVR2 | AACCAGTTCATGCTAACAGG | 4394 | |
TSWVF3 | CAGAAGCCATATCTATAAGTGG | 4001 | 2281 |
TSWVR3 | GGTGGATAGGAGAGCCAATG | 6282 | |
TSWVF4 | GGCAAAGACAGCTTCGAGAC | 6082 | 2832 |
TSWVR4 | AGAGCAATCAGGTACAACTAAAAC | 8914 |