Fig. 1

An aligned portion of the TGF-β domain. Primers that amplify both copies of amh were used to amplify a a male template and b a female template. c Male-specific amh primers and d Autosomal amh primers were used on a male template. Male-specific and autosomal variable sites are noted with arrows and the translated consensus is shown along the bottom. Image produced using Geneious v8.0.4 (http://www.geneious.com [12]), sequences are from 1873–1912 bp in KP686073 and 1871–1910 bp in KP686074