Fig. 1

Real-time reverse transcriptase polymerase chain reaction for detection of infectious bronchitis virus. cDNA derived from IBV Beaudette strain genomic RNA was diluted serially from 10⁶ to 10¹ copies/μl and detected by real-time PCR. The x-axis indicates the PCR cycle number, whereas the y-axis indicates the fluorescence intensity over the background. NC negative control, PCR polymerase chain reaction