Fig. 2
Agarose gel electrophoresis of conventional RT-PCR amplified products from infectious bronchitis virus N gene. Electrophoresis of PCR amplification after serial dilutions of the IBV Beaudette strain in a 1.5 % agarose gel stained with ethidium bromide. Lanes 1 and 10 50-bp DNA ladder; lane 2 negative control; lane 3 108 copies/μl; lane 4 107 copies/μl; lane 5 106 copies/μl; lane 6 105 copies/μl; lane 7 104 copies/μl; lane 8 103 copies/μl; lane 9 102 copies/μl. bp base pairs, IBV infectious bronchitis virus, RT-PCR reverse transcriptase-polymerase chain reaction