Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

Table 2 Intra- and inter-assay reproducibility of SYBR green-I-based real-time RT-PCR

Copy number	Mean crossing point	Intra-assay CV (%)	Inter-assay CV (%)
10¹	32.90	1.5	1.6
10²	28.25	1.2	1.8
10³	25.17	0.9	1.4
10⁴	21.84	0.7	1.2
10⁵	18.26	0.6	0.7
10⁶	16.63	0.6	0.6

ISSN: 1756-0500