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Fig. 2 | BMC Research Notes

Fig. 2

From: DAMe: a toolkit for the initial processing of datasets with PCR replicates of double-tagged amplicons for DNA metabarcoding analyses

Fig. 2

DAMe workflow. a DAMe initially sorts sequences by tag combination and collapses the repetitive sequences into unique sequences. An optional chimera removal step can be performed. With the resulting information a decision can be taken on the thresholds for filtering erroneous sequences prior to taxonomy assignment. Renkonen similarity index can be optionally computed for every pair-wise PCR replicate in order to test for the comparability of the PCR replicates. Also, sequences can optionally be de-collapsed if later taxonomy assignment requires this. b From each step different output files are obtained, containing information from each sequence such as the sample source, identified tag combination, frequency, and PCR reproducibility

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