Table 1 Summary of sequence abundance in the sorting and chimera removal steps of DAMe

Initial input

184,396 total sequences

718,848 total sequences

Sequences with errors in tag/primer

45,932 (24.9 % of total input)

119,619 (16.64 % of total input)

Total sorted sequences

138,464 (75.1 % of total input)

599,229 (83.36 % of initial input)

Total sorted unique sequences

29,952

47,489

Unique sequences from used tags

24,215 (80.85 % of total unique sorted sequences)

36,540 (76.9 % of total unique sorted sequences)

Unique sequences per used tag combination^a

11; 160.5; 198.5; 1087

1; 552.5; 609; 1896

Frequencies of unique sequences per used tag combination^a

1; 1; 4.8; 3245

1; 1; 15.47; 32,400

Unique sequences from unused tag combinations

5737 (19.15 % of total unique sorted sequences)

10,949 (23.1 % of total unique sorted sequences)

Unique sequences per unused tag combination^a

6; 59.5; 98.9; 592

1; 3; 4.7; 640

Frequencies of unique sequences per unused tag combination^a

1; 1; 3.67; 1740

1; 1; 3.09; 11,699

Chimeric sequences

634 (2.62 % of total unique sequences of used combinations)

1308 (3.6 % of total unique sequences from used combinations)

Unique chimeric sequences per used tag combination^a

0; 3; 5.2; 61

0; 10; 21.8; 149

Frequencies of unique chimeric sequences per used tag combination^a

1; 1; 1.36; 21

1; 1; 4.76; 661