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Table 2 Impact of the filtering thresholds on dataset 1

From: DAMe: a toolkit for the initial processing of datasets with PCR replicates of double-tagged amplicons for DNA metabarcoding analyses

Repr–CopyNum

Sorted clean

Y

y + t

y + t + la

2 PCRs, 2 times

23,336

2472

862

802

2 PCRs, 1 time

23,336

2472

2472

2348

1 PCR, 2 times

23,336

20,864

4195

3995

1 PCR, 1 time

23,336

20,864

20,864

19,647

  1. a y = minimum reproducibility; t = minimum copy number; l = minimum length