Fig. 1

Insert size distributions after second size selection. Data originate from analysis with Bioanalyzer instruments. a Insert size distributions of sequencing libraries obtained with the standard Illumina TruSeq^® DNA PCR-free LPP have an overall good reproducibility. All sequenced TS libraries are shown. b Modifications during DNA fragmentation and insert size selection enabled the creation of sequencing libraries with sharper and more symmetric insert size distributions. Sequencing libraries Efa_TS and Efa_IS1 are illustrated in red and blue, respectively. c In addition, different RB:BB ratios led to sequencing libraries varying in average insert size for the same genome. Blue: Pst_IS1, red: Pst_IS3. d Insert size distribution reproducibility is maintained when using modified LPPs