Fig. 2From: Optimized Illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assemblyLinear regression of Bioanalyzer deduced and actual average library insert sizes. Calculation of actual library insert sizes was done after remapping of raw read data to respective reference genome sequences. Linear regression analysis revealed a very strong correlation, but the Bioanalyzer system turned out to overestimate library insert sizesBack to article page