Fig. 4

Interplay between insert size, GC content and sequencing quality of a library. Insert size distributions of IS1-3 libraries were plotted prior to and after read quality filtering (80;20). Distributions marked green belong to IS1 libraries, whereas distributions marked blue are from higher category libraries (IS2 or IS3). Dark-coloured distributions are derived from unfiltered libraries. Light-coloured distributions are obtained after quality filtering. To make insert size distributions directly comparable to each other, read counts were normalized by the maximal read count (per insert size) of the unfiltered library (of same category and genome). a Bce_IS1 and Bce_IS3. b Efa_IS1 and Efa_IS2. c Sen_IS1 and Sen_IS2. d Mlu_IS1 and Mlu_IS3