Fig. 5

Effect of quality filtering on average library insert size. Average insert sizes for libraries Mlu₅₀_IS2, Mlu₅₀_IS3 and Mlu₅₀_IS4 were plotted as a function of progressive stringency filtering. Most relaxed stringency corresponds to unfiltered libraries (raw). During most discriminative filtering reads are only passing quality control, if at least 90 % of their nucleotides had a Phred quality score ≥20 (90;20)