Fig. 1From: Isolation and detection of microRNA from the egg of chickensProtocol for the generation of pooled miRNA. A flow-diagram detailing the generation of the pooled miRNA used for cDNA synthesis. A total of nine extractions from three biologically distinct homogenates (comprising of either the albumen or yolk of two eggs) is used to produce the three pools. The protocol is repeated in duplicateBack to article page