TY - JOUR AU - Van Acker, Nathalie AU - Ragé, Michael AU - Sluydts, Ellen AU - Knaapen, Michiel W. M. AU - De Bie, Martine AU - Timmers, Maarten AU - Fransen, Erik AU - Duymelinck, Carla AU - De Schepper, Stefanie AU - Anand, Praveen AU - Meert, Theo AU - Plaghki, Léon AU - Cras, Patrick PY - 2016 DA - 2016/05/23 TI - Automated PGP9.5 immunofluorescence staining: a valuable tool in the assessment of small fiber neuropathy? JO - BMC Research Notes SP - 280 VL - 9 IS - 1 AB - In this study we explored the possibility of automating the PGP9.5 immunofluorescence staining assay for the diagnosis of small fiber neuropathy using skin punch biopsies. The laboratory developed test (LDT) was subjected to a validation strategy as required by good laboratory practice guidelines and compared to the well-established gold standard method approved by the European Federation of Neurological Societies (EFNS). To facilitate automation, the use of thinner sections. (16 µm) was evaluated. Biopsies from previously published studies were used. The aim was to evaluate the diagnostic performance of the LDT compared to the gold standard. We focused on technical aspects to reach high-quality standardization of the PGP9.5 assay and finally evaluate its potential for use in large scale batch testing. SN - 1756-0500 UR - https://doi.org/10.1186/s13104-016-2085-4 DO - 10.1186/s13104-016-2085-4 ID - Van Acker2016 ER -