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Table 1 Primer sequences and amplification efficiency

From: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions

Target

Forward primer (5′–3′)

Reverse primer (5′–3′)

Amplicon (bp)

Reagent

Efficiency

GAPDH

ACCCACTCCTCCACCTTTGAC

TCCACCACCCTGTTGCTGTAG

110

ABI

0.98

TLR2

GTGATAGGTGTGAGGCAGGT

GTGGCCGCCTTGATTCATAG

136

ABI

0.93

CD1c

TTTCTGCAGTTTCTGCTGCTA

GAGACGTGTTCCTGGGATG

74

ABI

1.06

CD54

CCTTCCTCACCGTGTACTGG

AGCGTAGGGTAAGGTTCTTGC

90

ABI

1.05

CD68

TTCCCCTATGGACACCTCAG

TTGTACTCCACCGCCATGTA

86

ABI

1

CCL4

CCTGCTGCTTTTCTTACAC

CACAGACTTGCTTGCTTC

126

ABI

1.09

IL4

GTCTCACCTCCCAACTGCTT

GTTACGGTCAACTCGGTGCA

157

Bio rad

0.99

IL4

GTCTCACCTCCCAACTGCTT

GTTACGGTCAACTCGGTGCA

157

Roche

1.01

IL8

GAAGGAACCATCTCACTG

CCACTCTCAATCACTCTC

200

Bio rad

0.96

IL8

GAAGGAACCATCTCACTG

CCACTCTCAATCACTCTC

200

Roche

0.94

IL1RN

CCTGTCCTGTGTCAAGTCTGG

AGCGGATGAAGGCGAAGC

110

ABI

0.93

CEBPB

CGCTTACCTCGGCTACCA

ACGAGGAGGACGTGGAGAG

65

ABI

0.94

IL-18

TGCATCAACTTTGTGGCAAT

ATAGAGGCCGATTTCCTTGG

169

ABI

1

V-FOS

ACTACCACTCACCCGCAGAC

CCAGGTCCGTGCAGAAGT

75

ABI

0.98

Egr-1

AGCCCTACGAGCACCTGAC

GGTTTGGCTGGGGTAACTG

92

ABI

0.9

Egr-2

TTGACCAGATGAACGGAGTG

TGGTTTCTAGGTGCAGAGACG

121

ABI

0.92

CD43

AAGATGTCATCAGTGCCCCA

CACGGTGTGGGATCCTAGAG

90

ABI

0.93

CCR7

GGTGGTGGCTCTCCTTGTC

ACTGTGGTGTTGTCTCCGATG

84

ABI

1.1

CD40

GCAGGCACAAACAAGACTGA

ATGGCAAACAGGATCCCGAA

95

ABI

0.91

S01a

GGTACCGGGTCTCCACATGA

GGGAAAGTCACTCACCCAAGG

   

S01b

GTACCGGGTCTCCACCAGG

GGGAAAGTCACTCACCCAAGG

   

S03

CTTTTGCTTTCTGTTTCTTAAGGGC

TCAATCTTTGGGCAGGTTGAA

   

S04a

CTGGTGCCCACAGTTACGCT

AAGGATGCGTGACTGCTATGG

   

S04b

CTGGTGCCCACAGTTACGCT

AGGATGCGTGACTGCTCCTC

   

S05a

AAAGTAGACACGGCCAGACTTAGG

CATCCCCACATACGGAAAAGA

   

S05b

AGTTAAAGTAGACACGGCCTCCC

CATCCCCACATACGGAAAAGA

   

S06

CAGTCACCCCGTGAAGTCCT

TTTCCCCCATCTGCCTATTG

   

S07a

TGGTATTGGCTTTAAAATACTGGG

TGTACCCAAAACTCAGCTGCA

   

S07b

GGTATTGGCTTTAAAATACTCAACC

CAGCTGCAACAGTTATCAACGTT

   

S08a

CTGGATGCCTCACTGATCCA

TGGGAAGGATGCATATGATCTG

   

S08b

GCTGGATGCCTCACTGATGTT

TGGGAAGGATGCATATGATCTG

   

S09a

GGGCACCCGTGTGAGTTTT

TCAGCTTGTCTGCTTTCTGGAA

   

S09b

GGGCACCCGTGTGAGTTTT

CAGCTTGTCTGCTTTCTGCTG

   

S10a

GCCACAAGAGACTCAG

TGGCTTCCTTGAGGTGGAAT

   

S10b

TTAGAGCCACAAGAGACAACCAG

TGGCTTCCTTGAGGTGGAAT

   

S11a

TAGGATTCAACCCTGGAAGC

CCAGCATGCACCTGACTAACA

   

Hy

TTCTGGAACCTTTCTTTTCAGGC

ACTTCCCTCTGACATTACCTGATAATTG

   

HA-8p

TGCAGTCAGCAGATCACCC

CTTCTGGGCAACAGTTATGGA

   

KIR3 DS1

CATCRgTTCCATgATgCg

CCACgATgTCCAggggA

   

TCCATCggTCCCATgATgTT