Fig. 4
From: Simultaneous purification of DNA and RNA from microbiota in a single colonic mucosal biopsy
Comparison on taxonomic level of DNA purification using protocol 1 and commonly used DNA purification methods. The 16S sequence comparison bar charts are made using Classifier (http://rdp.cme.msu.edu/classifier/classifier.jsp). The significant differences of Firmicutes between paired libraries were calculated by the Library Compare Tool using a confidence threshold of 80 % (http://rdp.cme.msu.edu/comparison). Protocol 1: Mechanical pre-treatment only, followed by purification with AllPrep DNA/RNA Mini Kit as described in Additional file 1; DNA kit 1, 2 and 3: A combination of mechanical and enzymatic pre-treatments as recommended by Qiagen for lysis of Gram-positive bacteria, followed by purificaton with AllPrep DNA/RNA Mini Kit (kit 1), QIAamp DNA Stool Mini Kit (kit 2) and DNeasy Blood & Tissue Kit (kit 3) as described in Additional file 1. n number of clones sequenced