Simultaneous purification of DNA and RNA from microbiota in a single colonic mucosal biopsy

BMC Research Notes

Table 1 Purified DNA and RNA quantity and quality data and subsequent analysis

Comparison^a	Patient	Protocol	Quantity DNA (ng/µl)	Quality DNA (260/280)	Quantity RNA (ng/µl)	Quality RNA (RIN)	cDNA synthesis^b	Cloning and sequencing
Comparison^a	Patient	Protocol	Quantity DNA (ng/µl)	Quality DNA (260/280)	Quantity RNA (ng/µl)	Quality RNA (RIN)	cDNA synthesis^b	cDNA	DNA^c
1	Patient 1	Protocol 1	540	1.89	48.34	N/A			X
	Patient 1	Protocol 2	429	1.89	97.67	8.3	X (T)
	Patient 1	DNA kit1	258	1.90					X
	Patient 1	DNA kit2	353	1.88					X
	Patient 1	DNA kit3	219	1.88					X
	Patient 2	Protocol 1	366	1.88	38.95	N/A
	Patient 2	Protocol 2	200	1.92	85.12	8.5	X (T)
	Patient 2	DNA kit1	117	1.91
	Patient 2	DNA kit2	389	1.87
	Patient 2	DNA kit3	551	1.86
2	Patient 3	Qiazol, phase separation, kit column			140.53	8.5
	Patient 3	Protocol 2			109.82	8.7	X (T)
	Patient 3	Qiazol, phase separation, kit column			95.21	8.5
	Patient 3	Protocol 2			102.2	8.9	X (T)	X
3	Patient 4	Protocol 2	375	1.89	116.96	8.1			X (T)
	Patient 5	Protocol 2	521	1.88	56.26	7.3			X (T)
	Patient 4	Protocol 3	274	1.92	170.42	7			X (T)
	Patient 5	Protocol 3	353	1.90	102.27	7.3			X (T)

^aThree separate comparisons of purification protocols were performed due to limited number of biopsies
^bX = Sample being subjected to cDNA synthesis, T = Technical replicates
^cX = Sample being subjected to cloning and sequencing, T = Technical replicates

ISSN: 1756-0500