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Fig. 2 | BMC Research Notes

Fig. 2

From: Lentiviral gene transfer into human and murine hematopoietic stem cells: size matters

Fig. 2

Relationship between the viral RNA length and production of lentiviral particles. The quantity of lentiviral particles that are produced (calculated using RT-qPCR) plotted as a function of the viral RNA length (kb). Each point in the plot represents an individual virus batch, each time produced using the pLEGO-DEST backbone and the same transfection method and producer cell line (see Additional file 1: Supplementary Protocol). The viral RNA length is the length of the sequence flanked by the 5′ and 3′ SIN-LTRs in the lentiviral expression vector (see Fig. 1)

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