Fig. 3

Transduction efficiency of JURKAT, CD34⁺ human HSCs and murine Lin⁻ BM with lentiviral vectors of varying sizes. Transduction efficiency of different cell types as a function of the amount of virus particles, measured 4–6 days after transduction and expressed as the BFP⁺ percentage of the viable cells. Lentiviruses with three different viral RNA sizes (length between SIN-LTRs) are compared: 4215 (solid black lines), 5190 (dark-grey dashed lines) and 5750 bp (light-grey dashed lines). a Triplicate transductions ± SD of JURKAT cells using serial dilutions of each lentivirus, representative of one of three individually produced virus batches. Inset: example flow cytometry plot displaying the BFP⁺ transduced fraction. b Western blot probed with anti-2A antibody. Total lysates expressing the T2A-tagged proteins of the 4215 bp (BFP only, no T2A), 5190 and 5750 bp constructs, separated from BFP. (asterisk): non-specific signals. c Triplicate transductions ± SD of human CD34⁺ HSCs (solid triangles) and murine Lin⁻ BM (solid circles) with the three lentiviruses