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Fig. 4 | BMC Research Notes

Fig. 4

From: Lentiviral gene transfer into human and murine hematopoietic stem cells: size matters

Fig. 4

Transduction efficiency of JURKAT with independently generated and different lentiviral vectors of varying sizes. a Transduction efficiency of JURKAT cells as a function of the amount of virus particles, measured 4–6 days after transduction and expressed as the BFP+ percentage of the viable cells. Lentiviruses with three different viral RNA sizes (length between SIN-LTRs) are compared: 4215 (1xBFP; black), 5105 (2xBFP; blue) and 5995 bp (3xBFP; red). Triplicate transductions ± SD using serial dilutions of each lentivirus, representative of one of three individually produced virus batches. b Average transduction percentage of the different viruses, measured at the number of virus particles that yielded 75 % in the ‘1xBFP’ (4215 bp) vector (indicated by dashed vertical lines in Figs. 3 and 4a)

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