Fig. 2
The promoter of Exoenzyme S gene (PA3841) was activated when P. aeruginosa cells were in the biofilm state. Recombinants P. aeruginosa NT0 & NT3841P were grown at 37 °C in a 48-well microplate Bioflux system (Fluxion Biosciences, South San Francisco, CA) with a shear flow of 0.55 dyn/cm2 using 50 % BHI (Brain Heart Infusion) medium. Images were captured after 25- and 45-h growth using a confocal microscope (LSM710; Carl Zeiss MicroImaging, Thornwood, NY) with the excitation wavelength set at 488-nm. Left panels and right panels are representative fluorescence images of NT0 and NT3841P mutants respectively. The expression of GFP was increased in the biofilms formed by the NT3841P recombinant, but not in the biofilms formed by the control NT0 recombinant. Bars represent 10 µm