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Table 1 Characteristics of the 32 microsatellite markers developed for Distylium lepidotum

From: Novel microsatellite markers for Distylium lepidotum (Hamamelidaceae) endemic to the Ogasawara Islands

Locus

Repeat motif

Forward primer sequence (5′–3′)a

Reverse primer sequence (5′–3′)b

Ta (°C)

Size

range (bp)

GeneBank accession no.

Isu00524

(CT)30

[tail C] TTTATGCTTATTCACCCTTGAACC

gtttAAACACCCATTAGTTCTTCTGTCTG

57

136–194

LC085250

Isu01062

(TC)25

[tail B] TACGAATGATGGGTCAAACTGTAA

gtttGCCTTAAATTGACTGGAAGTGATT

57

228–270

LC085251

Isu01853

(AG)19

[tail D] CACTAGTTATTGAGGTAGGCGGGT

gTTTGTTAACGAATGAGTTGGGATT

57

274–302

LC085252

Isu03838

(TC)24

[tail D] TTCCTGAAACGGTTACACAATACA

gtttAGTGGAGATGATAAACGGATTGAC

57

111–135

LC085253

Isu04069

(GA)24

[tail B] TTAGATTTGAAGGCGATAAAGGTT

gttTCCTTGATCTGTCCAATGTCA

57

135–171

LC085254

Isu04385

(TC)22

[tail A] AATGGGTCAGTGAGAATCTGTCTT

gtttCAAGGAAATCGTATATGCAGAACA

57

215–245

LC085255

Isu04423

(GA)22

[tail B] AAGCAGAGCTTACCATGATTCACT

gttTAGATCTCTGAGGAGGGACACATT

57

260–308

LC085256

Isu04472

(AG)26

[tail D] ATTTGGATCATCACTCGAGGTAAA

gtTTATTCGTTTGCACTCTTATTTGA

57

214–266

LC085257

Isu04870

(CT)16

[tail B] TTAATTGGTTTCCCATTTGATCTC

gtttCATGCAGATGCAGACTCTAAGAAG

57

285–299

LC085258

Isu04950

(GA)22

[tail A] AGACAATTCTGTGCTCCAGTATCA

gtttAACATTGAAAGTTGAAGACCCAAC

57

263–299

LC085259

Isu04954

(TC)31

[tail A] CTAATCCAAATCAACCCATCTACG

gtttCACCTCTCGTTTACTTCCATTGAT

57

128–156

LC085260

Isu05730

(AT)11

[tail A] ACATCGTCACCTCTATTAACCGAC

gtttCAAGAGATTTCGAAGTGAAACAGA

57

346–366

LC085261

Isu06843

(AG)27

[tail B] GTTGACATCCCTACTCCTCCTACC

gttTCTAAGCAAATGTGCATCGTTAGT

57

96–132

LC085262

Isu07049

(CT)26

[tail A] TCCATGTATTTATTTCGATCCTCC

gtttGGGAAATACCATAAACATAAAGATGG

57

90–134

LC085263

Isu07063

(GA)24

[tail C] AGCTTGCATGAGGTTTCACTAAGA

gtttCGACAACAGTACTAATCAACACGG

57

109–143

LC085264

Isu09807

(GA)23

[tail D] AACGCAAGATTTATCATTACCAGC

gtttAAGACTCTCAAGATCTGTGCCAA

57

213–239

LC085265

Isu09853

(GA)22

[tail D] CAATTCCCTCAATTGTTGTTTCTT

gtttAGAAACTTAAAGACAAACCGGGAT

57

304–326

LC085266

Isu10193

(GA)24

[tail B] ATTTATGTGGAAGTAGTAGCCGGA

gttTACTGCTGGCTTGACATAGAAAGA

57

214–236

LC085267

Isu11459

(AG)19

[tail D] TAAAGCATCAAACAAGCGAATATG

gtttACAATAAGAAAGCGACATGCTCA

57

265–291

LC085268

Isu12115

(GA)11

[tail A] TACGATTCAAGCTTGTCATACTCG

gtttATATTTACGCGCAAACTCTCGC

57

413–417

LC085269

Isu12238

(CT)24

[tail D] CCAAGATTATGCAACCTAAGGAAG

gtttACCCTGAATTCCATCTAGACCTTT

57

116–156

LC085270

Isu12265

(TC)21

[tail C] TGATAGATACATGTCCCACTGTCTT

gttTAAACCTAGCCAAACAAATCCAAC

57

85–121

LC085271

Isu12586

(AT)11

[tail C] TAGACAACTTTCTGGATCAAAGCC

gtttGGCTGTGTATATGTATGCGTGTTT

57

319–359

LC085272

Isu13849

(CT)12

[tail D] CAAGATCAAGATTGAAATGGAATTG

gtttATCCGATAGATCAGTACTTGGTGG

57

326–350

LC085273

Isu13965

(AG)25

[tail B] GTGTAAGTTGTGGGTTTAACGGAT

gtttAAGACATCAGCAAACTAGTCCACC

57

155–183

LC085274

Isu15054

(TC)24

[tail A] CGGGATGTAAACATAGATGTCAAA

gttTATGGCCTAGGAAGATAATGTTGG

57

219–273

LC085275

Isu16246

(CT)26

[tail C] AATCATGTAGCGAGCTTGAACTTT

gtttCATGAATATGAGCACAAGGTATTATTT

57

132–174

LC085276

Isu16408

(TC)18

[tail C] AGATTACTGCTTCGTTCGACCTTA

gtTTGGTGCTATAATTAGGATTTGGC

57

285–307

LC085277

Isu16655

(CT)16

[tail C] GAAAGGTAGGTCCATAACTCCACA

gtTTGAGGATACAATGCTTTCACTTG

57

270–290

LC085278

Isu16805

(GA)26

[tail B] CGCTCTTAAACAGAATATGGAAGG

gtttGATTGTCAATTCCACGGAGAAC

57

83–115

LC085279

Isu17435

(AG)20

[tail B] TAAATACAAAGATGATGTGCCAGC

gttTGTACATGTAGTTCCCAGGCAAT

57

82–114

LC085280

Isu17619

(AG)13

[tail A] CAATTCCCTTGTGAAGAATTATCG

gtttGTTTACAGTACTGCACTGACGCAT

57

317–329

LC085281

  1. Ta = annealing temperature
  2. aTails of the forward primers are indicted as follows: [Tail A] = 5′-GCCTCCCTCGCGCCA-3′; [Tail B] = 5′-GCCTTGCCAGCCCGC-3′; [Tail C] = 5′-CAGGACCAGGCTACCGTG-3′; and [Tail D] = 5′-CGGAGAGCCGAGAGGTG-3′
  3. bReverse primer sequences contained the PIG-tail sequence [2]. Tail sequences are shown in lower case letters