Assessment of free fatty acids and cholesteryl esters delivered in liposomes as novel class of antibiotic

BMC Research Notes

Table 5 Hemolytic activity of the most potent antimicrobial lipids

Liposomal formulation	Hemolysis (% maximal hemolysis) at 900 μg/mL			At 90 μg/mL^a
Liposomal formulation	30 min	3 h	24 h	30 min	3 h	24 h
PA4	>40	>40	>40	1.61 ± 0.50	3.10 ± 1.00	70.43
SA4	>40	>40	>40	2.21 ± 1.80	4.04 ± 0.60	46.35
CL3	<20	>40	>40	1.13 ± 0.20	0.80 ± 0.10	54.55

Hemolysis was screened at 900 μg/mL lipid concentration and further tested at 90 μg/mL lipid concentration (mean ± S.D., n = 3 for 30 min and 3 h time points and n = 1 for 24 h time point). PA4 palmitic acid prepared in carrier-4, SA4 stearic acid prepared in carrier-4, CL3 cholesteryl linoleate prepared in carrier-3
^aThere were no statistically significant differences compared to control erythrocytes after 30 min and 3 h

ISSN: 1756-0500