Fig. 4

TLR10 expression in healthy equine tissues. a TLR10 expression determined by RT-qPCR. Samples from caudal mesenteric lymph nodes (MSN, n = 12), Bronchial lymph node (LN, n = 1), spleen (n = 2), lung (n = 1), colon (n = 4), kidney (n = 3) and liver (n = 3) were collected post slaughter/euthanasia and preserved in RNALater prior to RNA isolation, cDNA synthesis and RT-qPCR. TLR10 expression has been normalized to two house keeping genes, SDHA and HPRT and is expressed as 40-CT, mean is shown as horizontal line. ND = not determined (tissue not available) b TLR10 expression across different tissues in 3 individual horses where multiple tissue samples were available (these horses are also included in part A)