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Table 1 16S gene composition of the mock community

From: Pipeline for amplifying and analyzing amplicons of the V1–V3 region of the 16S rRNA gene

 

Strain

Genome sizea

# 16S genesa

Genome copies per microgramb

Reference or DSMZ catalogue numberc

1

Campylobacter jejuni 11168

1,641,481

3

5.64E+08

[23]

2

Salmonella enterica serovar Typhimurium SL1344

4,878,012

6

1.89E+08

[24]

3

Escherichia coli mg1655

4,656,144

7

1.99E+08

[25]

4

Megasphaera elsdenii LC-1

2,474,718

7

3.74E+08

DSM 20460

5

Cloacibacillus porcorum CL-84

3,585,187

3

2.57E+08

[26]

6

Brachyspira hyodysenteriae

3,050,489

1

3.04E+08

[27]

7

Haemophilus parasuis 29755

2,224,137

2

4.17E+08

[28]

8

Bordetella bronchiseptica 1289

5,207,899

3

1.78E+08

[29]

9

Staphylococcus aureus USA300

2,872,915

5

3.22E+08

[30]

10

Bacteroides thetaiotaomicron

6,293,399

5

1.47E+08

DSM 2079

11

Faecalibacterium prausnitzii A2-165

3,080,849

3

3.01E+08

DSM 17677

12

Streptococcus parasanguinis

2,153,652

4

4.30E+08

DSM 6778

13

Parabacteroides merdae

4,431,877

3

2.09E+08

DSM 19495

14

Oscillibacter valericigenes

4,470,622

3

2.07E+08

DSM 18026

15

Desulfovibrio gigas

3,788,225

4

2.45E+08

DSM 1382

16

Lactobacillus delbrueckii subspecies bulgaricus

1,864,998

9

4.97E+08

DSM 20081

17

Coriobacterium glomerans

2,115,681

2

4.38E+08

DSM 20642

18

Oxalobacter formigenes BA-2

2,509,362

1

3.69E+08

DSM 4420

19

Roseburia hominis A2-183

3,592,125

4

2.58E+08

DSM 16839

20

Methanobrevibacter smithii d

1,704,865

1

5.43E+08

DSM 2375

  1. aAll except C. porcorum were calculated by the Joint Genome Institute’s Integrated Microbial Genomes Database https://img.jgi.doe.gov/cgi-bin/w/main.cgi. C. porcorum was calculated manually (BioProject PRJNA335387)
  2. bEstimates were calculated by URI Genomics & Sequencing Center http://cels.uri.edu/gsc/cndna.html
  3. cGenomic DNAs were acquired from DSMZ, the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures https://www.dsmz.de/home.html
  4. dThis archaeon was included as a control for non-specific amplification