From: Pipeline for amplifying and analyzing amplicons of the V1–V3 region of the 16S rRNA gene
Mock community | Actual number of OTUsa | Observed number of OTUs | Estimated total number of OTUsb | Chao diversity index | Shannon diversity index | Inverse Simpson index | Error rate (%) | File size (Gb)c |
---|---|---|---|---|---|---|---|---|
All sequences | 20 | 734 ± 56 | 374,770 ± 214,807 | 21,676 ± 3273 | 3.6 ± 0.1 | 18 ± 0.8 | 3.6 | 41 |
Singletons removed | 20 | 28 ± 0.8 | 68 ± 13 | 41 ± 3 | 2.7 ± 0.02 | 12 ± 0.3 | 1.4 | 21 |
Single and doubletons removed | 20 | 22 ± 0.3 | 22 ± 0.3 | 23 ± 0.7 | 2.6 ± 0.02 | 12 ± 0.3 | 1.3 | 3 |