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Fig. 2 | BMC Research Notes

Fig. 2

From: Penicillin binding protein 3 of Staphylococcus aureus NCTC 8325-4 binds and activates human plasminogen

Fig. 2

Binding of cell-free growth medium of the FTP library clones to immobilized plasminogen. a Binding of clarified growth medium from 95 representative FTP library clones to immobilized plasminogen is shown. The clone encoding a fragment of S. aureus PBP3 is named ΔPBP3. Clarified growth medium of the 96th clone, the host strain MKS12 carrying the empty vector pSRP18/0, was used as the background level and the value obtained with this control was subtracted from the other results. The results were measured in ELISA plate reader at A405nm. b Binding of the clarified growth medium from the ΔPBP3 library clone to immobilized plasminogen is shown. Purified, His-tagged enolase of Lactobacillus crispatus ST1 (ST1 Eno) represents a positive control protein and the clarified supernatant from the clone named ΔArcB, encoding a fragment of S. aureus ArcB protein, represents the negative control. The binding of clarified supernatant from MKS12 (pSRP18/0), named as Control, is also shown. The results were measured in ELISA plate recorder at A405nm. The mean and ± SD of duplicate samples from a representative experiment are shown

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