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Fig. 4 | BMC Research Notes

Fig. 4

From: Penicillin binding protein 3 of Staphylococcus aureus NCTC 8325-4 binds and activates human plasminogen

Fig. 4

Binding of soluble plasminogen to His-tagged recombinant PBP3 proteins. The binding of soluble plasminogen to immobilized a PBP3-CS634 and b PBP3-F and PBP3-C was measured with VICTOR® time-resolved fluorometer as counts per second (cps) at 340/615 nm. Laminin (Lam) and ΔNarG were included as controls. The test included binding in the presence of the proteins to be tested and (i) Plg (black bars, Plg), (ii) Plg and EACA (grey bars, EACA), (iii) binding in the absence of Plg or EACA (white bars, w/o). The mean and the ±SD of duplicate samples of one representative experiment are shown. SDS-PAGE analysis of the purified PBP3-CS634, PBP3-F and PBP3-C recombinant proteins are shown above the respective fluorometry results in figure a and b

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