Fig. 5
Enhancement of plasmin formation by His-tagged recombinant PBP3 proteins. The activation of plasminogen to plasmin by PBP3-F, PBP3-C, PBP3-CS634, Lam and ΔNarG was measured in ELISA plate recorder (A405nm) at 15–30 min time intervals for 4.5 h. The background level with buffer only (Bfr) is also shown. The test included plasminogen activation by the corresponding protein (PBP3-F, PBP3-C, PBP3-CS634, Lam, ΔNarG) or buffer only (Ctrls) in the presence of (i) Plg, tPA and the substrate, (ii) Plg, tPA, EACA and the substrate (EACA), (iii) Plg and the substrate without tPA (w/o tPA), iv) tPA and the substrate without Plg and (v) substrate in buffer. The mean and ±SD of the duplicate samples from one representative experiment are shown