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Fig. 1 | BMC Research Notes

Fig. 1

From: Development of a high throughput drug screening assay to identify compounds that protect oligodendrocyte viability and differentiation under inflammatory conditions

Fig. 1

IFNγ reduces OL viability and inhibits differentiation. A Expanded OPCs were plated in 96 well plates in DMEM differentiation media for 24 h and either treated for 1 h with 1.1 µM quetiapine (QTP) or vehicle (0.1 % DMSO), followed by 48 h of 200 U/ml INFγ insult. No Insult was vehicle treated with no IFNγ insult. OLs were immunostained for MBP (green) and nuclei stained with DAPI (blue). Bar 200 μM. B 24 h after plating, differentiating OLs were treated with increasing concentrations of INFγ for 48 h. alamarBlue® (AB) fluorescence was quantified to determine cell viability. C 24 h after plating, differentiating OLs were treated with increasing concentrations of QTP for 1 h followed by 48 h treatment with 200 U/ml IFNγ. Cell viability was measured by quantification of alamarBlue® fluorescence. Image quantification of anti-MBP immunostaining of the same cultures was used to measure OL differentiation

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