Fig. 3

Analysis of the acute OL differentiation screen of the NCC compound library. a Dot plot representation of the entire high-throughput screening data set used to identify promoters of OL differentiation. Highlighted are the primary hit compound (red) activity. The mean response is indicated by the blue solid line. The black dotted line delineates the value of three standard deviations above the mean. The red dotted line delineates the ≥50 % positive control selection criteria. b Scatter plot representation of the high-throughput screening assay window. Using the T3/DMSO ratio as the window of measurable OL differentiation, the ratio of the positive (T3) to negative (DMSO) controls is depicted. The red line delineates the mean average ratio value for the entire NCC library screen = 3.1. The numbers above the scatter indicate the coefficient of variation (CV) for each plate. CV values <20 ± 5 were considered in the acceptable range. Each point is an average value from 16 fields. Each dot cluster represents eight control ratios from each NCC plate (n = 8, mean ± SEM). c Average raw values of DMSO and T3 for each library screening plate. Inset, The statistical analysis of the raw values of controls from all plates of the entire NCC library screen. d Summary of hit selection from the NCC library screen