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Fig. 2 | BMC Research Notes

Fig. 2

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

Fig. 2

RNA transcript levels of six target genes from adult and cord blood samples. RNA isolated from adult and cord blood samples using five different protocols and analyzed by qPCR. a Relative transcript levels of six genes from adult blood samples collected in Tempus tubes (n = 11–12 Tempus tubes for each protocol). b Relative transcript levels of six genes from cord blood samples collected in Tempus tubes (n = 3–6 Tempus tubes for each protocol). The Tempus 6-port samples were used as reference samples (calibrators) and all other samples were compared against the reference samples. Each bar represents the average log2-transformed fold change values; fold change = 2−∆∆Cq. The error bars indicate ± SE and the stippled lines indicate ± twofold

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