Fig. 2From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobankRNA transcript levels of six target genes from adult and cord blood samples. RNA isolated from adult and cord blood samples using five different protocols and analyzed by qPCR. a Relative transcript levels of six genes from adult blood samples collected in Tempus tubes (n = 11–12 Tempus tubes for each protocol). b Relative transcript levels of six genes from cord blood samples collected in Tempus tubes (n = 3–6 Tempus tubes for each protocol). The Tempus 6-port samples were used as reference samples (calibrators) and all other samples were compared against the reference samples. Each bar represents the average log2-transformed fold change values; fold change = 2−∆∆Cq. The error bars indicate ± SE and the stippled lines indicate ± twofoldBack to article page