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Table 1 Overview of the five RNA isolation protocols

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

RNA isolation protocol RNA isolation kit name RNA processing method Technologies for tot RNA extraction Simultenous isolation of all RNA speciesc
MagMAX semi-automateda MagMAX™ for Stabilized Blood
Tubes RNA Isolation Kit, compatible with Tempus™ Blood
RNA Tubes
Semi-automated Magnetic beads based RNA purification system Yes
MagMAX manuala MagMAX™ for Stabilized Blood
Tubes RNA Isolation Kit, compatible with Tempus™ Blood
RNA Tubes
Manual Magnetic beads based RNA purification system Yes
Norgenb Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes) Manual Column-based RNA purification systems:resin as the separation matrix Yes
Tempus Spina Tempus™ Spin RNA Isolation Kit Manual Column-based RNA purification systems:silica membrane No
Tempus 6-porta Tempus™ 6-Port RNA Isolation Kit Semi-automated Column-based RNA purification systems:silica membrane No
  1. aThe kit supplier is Life Technologies, Norway
  2. bThe kit supplier is Norgen Biotek Corp, Canada
  3. cAll RNA species can be simultaneously isolated according to the manufacturer’s protocol