Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

Table 1 Overview of the five RNA isolation protocols

RNA isolation protocol	RNA isolation kit name	RNA processing method	Technologies for tot RNA extraction	Simultenous isolation of all RNA species^c
MagMAX semi-automated^a	MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes	Semi-automated	Magnetic beads based RNA purification system	Yes
MagMAX manual^a	MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes	Manual	Magnetic beads based RNA purification system	Yes
Norgen^b	Preserved Blood RNA Purification Kit I (for use with Tempus Blood RNA Tubes)	Manual	Column-based RNA purification systems:resin as the separation matrix	Yes
Tempus Spin^a	Tempus™ Spin RNA Isolation Kit	Manual	Column-based RNA purification systems:silica membrane	No
Tempus 6-port^a	Tempus™ 6-Port RNA Isolation Kit	Semi-automated	Column-based RNA purification systems:silica membrane	No

^aThe kit supplier is Life Technologies, Norway
^bThe kit supplier is Norgen Biotek Corp, Canada
^cAll RNA species can be simultaneously isolated according to the manufacturer’s protocol

ISSN: 1756-0500