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Table 2 Comparison of RNA QC using five RNA isolation protocols

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

RNA isolation protocol RIN value OD 260/280 ratio OD 260/230 ratio Number of Tempus tubes
(a) Adult blood
 MagMAX semi-automated 7.85 ± 0.13 2.09 ± 0.01 1.97 ± 0.05 12
 MagMAX manual 7.20 ± 0.11 2.12 ± 0.01 1.19 ± 0.12* 12
 Norgen 7.36 ± 0.12 2.12 ± 0.02 1.76 ± 0.07* 12
 Tempus spin 8.97 ± 0.05 2.09 ± 0.01 2.08 ± 0.01 12
 Tempus 6-port 8.38 ± 0.08 2.20 ± 0.06 2.02 ± 0.05 11
 Average 7.93 ± 0.10 2.12 ± 0.01 1.80 ± 0.05 59
(b) Cord blood
 MagMAX semi-automated 7.47 ± 0.22 2.09 ± 0.03 2.01 ± 0.09 3
 MagMAX manual 7.73 ± 0.47 2.12 ± 0.01 2.14 ± 0.02 3
 Norgen 8.23 ± 0.33 2.11 ± 0.01 2.07 ± 0.04 3
 Tempus spin 7.13 ± 0.13 2.10 ± 0.01 2.11 ± 0.01 3
 Tempus 6-port 8.37 ± 0.38 2.10 ± 0.01 2.10 ± 0.02 6
 Average 7.88 ± 0.19 2.10 ± 0.02 2.09 ± 0.05 18
  1. * RNA extracted with MagMAX manual and Norgen protocols have significantly lower OD 260/230 ratio compared to RNA from the other protocols, p < 0.05