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Fig. 3 | BMC Research Notes

Fig. 3

From: Immunolocalization of pectic polysaccharides during abscission in pea seeds (Pisum sativum L.) and in abscission less def pea mutant seeds

Fig. 3

Light micrographs of sections stained with toluidine blue to show structural differences (a, f, k and p) and micrographs of indirect immunofluorescence detection of pectic epitopes in the AZ of dwarf wild type (JI2822) and ALZ of dwarf def mutant (JI3020) pea seeds. a–e Dwarf wild type young seeds (JI2822). f–j Dwarf def mutant young seed (JI3020). k–o Dwarf wild type mature seed (JI2822). p–t Dwarf def mutant mature seed (JI3020). b, g, l and q Immunolabeling with LM5 galactan. c, h, m and r Immunolabeling with LM6 arabinan. d, i, n and s Immunolabeling with JIM5. e, j, o and t Immunolabeling with JIM7. PL palisade layer; CPL counter palisade layer; AZ abscission zone; FN funicle; SE seed; ALZ abscission less zone. Arrows indicated the AZ. Scale bars 100 μm

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BMC Research Notes

ISSN: 1756-0500

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