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Fig. 5 | BMC Research Notes

Fig. 5

From: Immunolocalization of pectic polysaccharides during abscission in pea seeds (Pisum sativum L.) and in abscission less def pea mutant seeds

Fig. 5

Micrographs of indirect immunofluorescence detection of pectic epitopes LM5 galactan and LM6 arabinan in F3 population from cross of dwarf wild type JI 2822 and tall def mutant JI 1184 at pod number 2 stage (a–f) and F3 population from cross of the dwarf wild type JI 2822 and dwarf def mutant JI 3020 (g–p). a–c and g–k Immunolabelling with LM5 galactan. d–f and l–p Immunolabelling with LM6 arabinan. a and d Homozygous dominant (Def/Def) F3 line 11 showing the presence of double palisade layers as in the wild type phenotype. b and e Homozygous recessive (def/def) F3 line 18 showing the presence of the ALZ as in def mutant phenotypes. c and f Heterozygous (Def/def) F3 line 14 showing the presence of PL and CPL. g and l Homozygous dominant (Def/Def) F3 line 1 showing the presence both PL and CPL as in the WT phenotypes. h and m Homozygous recessive (def/def) F3 line 33 showing the ALZ as in parents def mutant phenotypes. i and p Heterozygous (Def/def) F3 line 77. i and n Young seed at pod stage 3.1. j and o Intermediated seed at pod stage 2.1. k and p Mature seed at pod stage 1.1. PL palisade layer; CPL counter palisade layer; AZ abscission zone; FN funicle; SE seed. Scale bars 50 μm

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