Fig. 2From: Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virusNA RT-PCR product. a Schematically, the designed primer sets generate overlapping fragments (blue bars) to obtain the full length of NA gene (red bars). Black bars represent the untranslated region (UTR). The black arrows represent the inner primers for sequencing to obtain complete coding sequences. b The NA1 and NA2 fragments were approximately 700 bp in length. NC represents negative control. Line 1, 2, and 3 represent the annealing temperatures of 50, 55 and 60 °C, respectively. M represents DNA Marker with size increment of 100 bpBack to article page