From: Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus
Fragment | Primer | Sequence (5′-3′) | Location | Tm (°C) |
---|---|---|---|---|
HA1 | HA-1Fa | CTCGAGAGCAAAAGCAGGGG | 5′ end | 65.62 |
HA269F | CTCAGTGTGATGGCTTCCAA | 269–288 |  | |
HA465 R | GTTATTAGATCTCCTTATG | 465–483 |  | |
HA907Ra | GGTTTGTCATTGGGAATGCT | 907–926 | 61.22 | |
HA2 | HA828Fa | ACGAAGTGGGAAAAGCTCAATA | 828–849 | 62.31 |
HA1266F | TCAGGACCTTGAGAAATATGTTG | 1266–1288 |  | |
HA + 1778Ra | AGTAGAAACAAGGGTGTTTT | 3′ end | 57.15 | |
NA1 | NA-1Fa | GAGCAAAAGCAGGAGTAAAG | 5′ end | 59.13 |
NA243F | AGCAGAATACAGAAATTGGTC | 243–263 |  | |
NA385R | GTCAGGATCGCATGACACAT | 385–406 |  | |
NA787Ra | TGACAATGTGCTAGTATGAAC | 787–807 | 58.05 | |
NA2 | NA636Fa | AGATAGTGTTGTTTCATGGTC | 636–656 | 57.96 |
NA989F | ACAGCTCCAGCAGTAGCCATTG | 989–1010 |  | |
NA + 1413Ra | AGTAGAAACAAGGAGTTTTT | 3′ end | 54.63 |