Fig. 3From: Infrared-emitting, peptidase-resistant fluorescent ligands of the bradykinin B2 receptor: application to cytofluorometry and imagingCytofluorometric detection of B-10665 (1 nM) in detached HEK 293a cells stably expressing human myc-tagged B2Rs and competition of binding by the alternate antagonist icatibant. a Sample histograms for infrared fluorescence. Detached cells were treated for 30 min with B-10665; icatibant was applied 15 min before B-10665 when utilized. b Concentration-effect relationship for cell labeling with B-10665 and competition with icatibant in replicated experimentsBack to article page