Fig. 6

Imaging of the B₂R distribution in a plate of wells containing HEK 293a cells that stably express myc-tagged recombinant human or rat receptors (hu myc-B₂R, rt myc-B₂R, respectively) or non-transfected cells (nt). a Infrared fluorescence following treatment with B-10665 (30 min, 37 °C, followed by ample rinsing). The color signal represents infrared fluorescence intensity, as quantified on the scale at the right hand side, and is overlayed on a black and white photograph of the object. Top fluorescence recorded during the first exposure (60 s); bottom results from the second exposure of the same object (60 s). b Detection of the receptors via immunohistochemistry of their myc tag (blue precipitate). The cell wells were submitted to the same treatments as in a. In both a and b, results are representative of 2 separate experiments