Fig. 1

Detection of SAM synthesized from Met and ATP as substrates by the MAT-catalyzed reaction over time. The cELISA was used to measure SAM. The samples for the cELISA were the product of the following biochemical reaction. MAT was added to a maximum of 2 mM Met and ATP in the buffer described in the Methods at 37 °C. The biochemical reaction and ELISA were performed simultaneously and then stopped at 20, 30, 40, 50, 60, 70 and 80 min after the reaction. Different amounts of MAT and buffers were tested