Fig. 3
Standard curves for the mouse monoclonal antibody against SAM in the sensitivity tests. a Using antibody 84-3 at 1:35,000 with different amounts of coating antigens (0.1, 0.15, and 0.2 µg/mL) in an indirect cELISA. b Using antibody 118-6 at 1:32,000 with coating antigen at 0.1 µg/mL in an indirect cELISA. The x-axis shows the values of the base 10 logarithm of the standard concentrations. The y-axis is expressed as LOGIT, which is defined as Ln(A/AS0/(1 − A/AS0)), where A is the OD450 value of the standard and AS0 is the OD450 value of the blank (without antigen). The equation for each standard curve is shown