Fig. 4

Cross-reactivity of the mouse anti-S-adenosylhomocysteine monoclonal antibody 301-1 with SAH analogs. Each well of a 96-well plate was coated with 0.5 µg/mL BSA-SAH. Serial dilutions of SAH standard (SAH sodium, SAH-Na), S-adenosylmethionine (SAM4: from Sigma-Aldrich Cat# A2408, SAM1: aza-SAM), homocysteine (H-Cys), l-cysteine (L-Cys), adenosine (Ade), glutathione (GST), L-cystathionine (L-CTT), methylthioadenosine (MTA), ADP (adenosine diphosphate), ATP (adenosine triphosphate) and properly diluted 301-1 were added. HRP-conjugated goat anti-mouse IgG antibody and TMB substrate were added to develop the color, and the OD450 was measured. The y-axis shows the OD450 for each test. The x-axis shows the concentration of SAH. A lower OD450 value indicates greater competition