Skip to main content

Table 2 Sensitivity of mouse anti-SAM antibody 118-6

From: Immunoassay of S-adenosylmethionine and S-adenosylhomocysteine: the methylation index as a biomarker for disease and health status

SAM standard (nM)

OD450

OD450

OD450

Mean

SD

Blank

0.0556

0.0808

0.0616

0.0660

0.0131

0

1.2189

1.2831

1.3030

1.2683

0.0439

6.3

1.110

1.0648

0.9757

1.0502

0.0683

12.5

0.885

0.8004

0.8737

0.8530

0.0459

25

0.6252

0.6435

0.6108

0.6265

0.0164

50

0.3838

0.3995

0.3782

0.3872

0.0110

Sensitivitya

2.6 nM

  1. aSensitivity was estimated by the corresponding concentration calculated from standard curve given the OD 450 (=OD450 at zero antigen – 2 × standard deviation of blank wells). ELISA was performed in triplicates. PLL-aza-SAM was coated at 0.1 µg/mL and the 118-6 used at 1:32,000
Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us