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Table 6 Microarray and qRT-PCR expression of genes chosen for validation

From: Differential expression of genes related to gain and intake in the liver of beef cattle

  qRT-PCR Microarray
Group Xa Group Ya Fold changeb Nominal P value Group Xa Group Ya Fold changeb Nominal P value
AGPAT3 0.039 0.099 1.15 0.53 10.63 11.38 1.68 0.03
DNAJB2 0.094 −0.099 −1.56 0.64 8.8 8.22 −1.49 0.04
FABP3 −0.32 −0.019 2.00 0.008 6.75 7.47 1.65 0.02
IGFBP1 0.12 −0.37 −3.09 0.29 15.48 11.84 −12.47 0.009
NAT1 −0.49 −0.31 1.51 0.54 10.02 10.74 1.65 0.03
NCAPG 0.67 0.26 −2.57 0.59 6.21 5.77 −1.36 0.008
PLK3 −0.18 −0.02 1.45 0.06 7.02 7.41 1.31 0.03
PPP1CA −0.021 0.1 1.32 0.37 10.19 10.54 1.27 0.05
PTK2B 0.054 −0.16 −1.64 0.18 7.05 7.67 1.54 0.03
SMAD6 0.16 0.11 −1.12 0.15 7.13 7.74 1.53 0.001
TAP2 0.12 0.26 1.38 0.39 9.11 10.02 1.88 0.005
TIGAR −0.18 0.09 1.86 0.09 7.54 7 −1.45 0.02
  1. aFor AGPAT3, DNAJB2, SMAD6, and TAP2, Group X was high gain-low intake and Group Y was low gain-high intake. FABP3, PTK2B, PLK3, and TIGAR had high gain-high intake (Group X) and low gain-high intake (Group Y). IGFPB1, NAT1, and NCAPG had high gain-low intake (Group X) and low gain-low intake (Group Y). PPP1CA had high gain-high intake (Group X) and low gain-low intake (Group Y)
  2. bExpression of Groups X and Y (the groups with the greatest differential expression) were compared to determine fold change for each gene