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Table 6 Microarray and qRT-PCR expression of genes chosen for validation

From: Differential expression of genes related to gain and intake in the liver of beef cattle

 

qRT-PCR

Microarray

Group Xa

Group Ya

Fold changeb

Nominal P value

Group Xa

Group Ya

Fold changeb

Nominal P value

AGPAT3

0.039

0.099

1.15

0.53

10.63

11.38

1.68

0.03

DNAJB2

0.094

−0.099

−1.56

0.64

8.8

8.22

−1.49

0.04

FABP3

−0.32

−0.019

2.00

0.008

6.75

7.47

1.65

0.02

IGFBP1

0.12

−0.37

−3.09

0.29

15.48

11.84

−12.47

0.009

NAT1

−0.49

−0.31

1.51

0.54

10.02

10.74

1.65

0.03

NCAPG

0.67

0.26

−2.57

0.59

6.21

5.77

−1.36

0.008

PLK3

−0.18

−0.02

1.45

0.06

7.02

7.41

1.31

0.03

PPP1CA

−0.021

0.1

1.32

0.37

10.19

10.54

1.27

0.05

PTK2B

0.054

−0.16

−1.64

0.18

7.05

7.67

1.54

0.03

SMAD6

0.16

0.11

−1.12

0.15

7.13

7.74

1.53

0.001

TAP2

0.12

0.26

1.38

0.39

9.11

10.02

1.88

0.005

TIGAR

−0.18

0.09

1.86

0.09

7.54

7

−1.45

0.02

  1. aFor AGPAT3, DNAJB2, SMAD6, and TAP2, Group X was high gain-low intake and Group Y was low gain-high intake. FABP3, PTK2B, PLK3, and TIGAR had high gain-high intake (Group X) and low gain-high intake (Group Y). IGFPB1, NAT1, and NCAPG had high gain-low intake (Group X) and low gain-low intake (Group Y). PPP1CA had high gain-high intake (Group X) and low gain-low intake (Group Y)
  2. bExpression of Groups X and Y (the groups with the greatest differential expression) were compared to determine fold change for each gene
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BMC Research Notes

ISSN: 1756-0500

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