Phytochemical analysis | Collection sites | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Chemical constituents | Test reagent | Observation | 1. | 2. | 3. | 4. | 5. | 6. | 7. | 8. | 9. | 10. | 11. | 12. |
Alkaloids | Mayer’s reagent | White ppt | +++ | +++ | +++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | + | ++ |
Glycosides | 10% Lead acetate solution | White ppt | +++ | ++ | +++ | ++ | ++ | +++ | ++ | +++ | +++ | ++ | ++ | ++ |
Reducing sugar | Benedict’s solution | Reddish brown ppt | ++ | ++ | ++ | +++ | ++ | ++ | +++ | ++ | ++ | +++ | ++ | +++ |
Phenolic compounds | Ferric chloride solution | Green colour | +++ | +++ | +++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Steroids and terpenoids | Acetic anhydride and conc: H2SO4 | Pink colour | ++ | ++ | + | ++ | ++ | + | + | + | ++ | + | +++ | ++ |
Flavonoids | Benzene FeCl3 | Yellow ppt | +++ | +++ | +++ | +++ | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ |
Tannins | FeCl3 and 10% lead acetate | White ppt | ++ | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | + | ++ | + |
Saponin glycosides | Distilled water | Frothing take place | +++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |