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Fig. 1 | BMC Research Notes

Fig. 1

From: Arginase-I enhances vascular endothelial inflammation and senescence through eNOS-uncoupling

Fig. 1

Overexpression of Arg-I promotes endothelial senescence and inflammation. The non-senescent endothelial cells (passage 2 to 4) were transduced with empty rAd/CMV vector as control (con) or rAd/CMV-Arg-I (Arg-I). Forty-eight hours post-transduction, the cells were serum-starved for 16 h, the cell lysates were then prepared and subjected to immunoblotting analysis of a Arg-I, expression of endothelial inflammation markers VCAM-1 and ICAM-1, and senescence markers p53-S15, p53 and CDK inhibitor p21Cip1 levels; b Arg-I and Arg-II expression. Bar graphs in the right panels show quantification of the signals. Tubulin served as loading control. ***p < 0.005 vs. control (con). n.s. not significant

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